| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human TNFSF14 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
TNFSF14 |
| Gene ID: |
8740 |
| Uniprot ID: |
TNF14_HUMAN |
| Specificity: |
This kit recognizes Human TNFSF14 in samples. No significant cross-reactivity or interference between Human TNFSF14 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines. |
| Post Translational Modifications | N-glycosylated. The soluble form of isoform 1 derives from the membrane form by proteolytic processing. |
| Function | Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Acts as a ligand for TNFRSF14/HVEM. Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production. |
| Protein Name | Tumor Necrosis Factor Ligand Superfamily Member 14Herpes Virus Entry Mediator LigandHvem-LHerpesvirus Entry Mediator LigandCd Antigen Cd258 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 14 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 14 - Soluble Form |
| Database Links | Reactome: R-HSA-5668541Reactome: R-HSA-5669034Reactome: R-HSA-5676594 |
| Cellular Localisation | Tumor Necrosis Factor Ligand Superfamily Member 14Membrane Form: Cell MembraneSingle-Pass Type Ii Membrane ProteinSoluble Form: SecretedIsoform 2: Cytoplasm |
| Alternative CLIA Names | Tumor Necrosis Factor Ligand Superfamily Member 14 CLIA kitHerpes Virus Entry Mediator Ligand CLIA kitHvem-L CLIA kitHerpesvirus Entry Mediator Ligand CLIA kitCd Antigen Cd258 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 14 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 14 - Soluble Form CLIA kitTNFSF14 CLIA kitHVEML CLIA kitLIGHT CLIA kitUNQ391 CLIA kitPRO726 CLIA kit |
| Specificity | This kit recognizes Human TNFSF14 in samples. No significant cross-reactivity or interference between Human TNFSF14 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-98 | 93 |
| EDTA plasma(n=8) | 93-107 | 101 |
| Cell culture media(n=8) | 89-100 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 43.42 | 78.25 | 311.28 | 43.07 | 75.80 | 315.52 |
| Standard deviation | 5.31 | 7.90 | 23.69 | 4.67 | 6.24 | 21.77 |
| CV (%) | 12.23 | 10.10 | 7.61 | 10.84 | 8.23 | 6.90 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
