| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This TNFSF13 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
TNFSF13 |
| Gene ID: |
8741 |
| Uniprot ID: |
TNF13_HUMAN |
| Specificity: |
This kit recognizes Human TNFSF13 in samples. No significant cross-reactivity or interference between Human TNFSF13 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The precursor is cleaved by furin. |
| Function | Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. |
| Protein Name | Tumor Necrosis Factor Ligand Superfamily Member 13A Proliferation-Inducing LigandAprilTnf- And Apol-Related Leukocyte Expressed Ligand 2Tall-2Tnf-Related Death Ligand 1Trdl-1Cd Antigen Cd256 |
| Database Links | Reactome: R-HSA-450520Reactome: R-HSA-5669034 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Tumor Necrosis Factor Ligand Superfamily Member 13 ELISA kitA Proliferation-Inducing Ligand ELISA kitApril ELISA kitTnf- And Apol-Related Leukocyte Expressed Ligand 2 ELISA kitTall-2 ELISA kitTnf-Related Death Ligand 1 ELISA kitTrdl-1 ELISA kitCd Antigen Cd256 ELISA kitTNFSF13 ELISA kitAPRIL ELISA kitTALL2 ELISA kitZTNF2 ELISA kitUNQ383 ELISA kitPRO715 ELISA kit |
| Specificity | This kit recognizes Human TNFSF13 in samples. No significant cross-reactivity or interference between Human TNFSF13 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-106 | 97 |
| EDTA plasma (n=8) | 90-100 | 95 |
| Cell culture media (n=8) | 92-105 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 242.40 | 597.95 | 1739.30 | 266.54 | 555.38 | 1628.44 |
| Standard deviation | 14.11 | 26.07 | 91.14 | 15.46 | 32.27 | 80.12 |
| CV (%) | 5.82 | 4.36 | 5.24 | 5.80 | 5.81 | 4.92 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
