Applications: |
CLIA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
4.69pg/mL |
Detection Limit: |
7.81~500pg/mL |
Short Description: |
This human TNFSF10 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
TNFSF10 |
Gene ID: |
8743 |
Uniprot ID: |
TNF10_HUMAN |
Specificity: |
This kit recognizes Human TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Human TRAIL/TNFSF10 and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Tissue Specificity | Widespread.most predominant in spleen, lung and prostate. |
Post Translational Modifications | Tyrosine phosphorylated by PKDCC/VLK. |
Function | Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. |
Protein Name | Tumor Necrosis Factor Ligand Superfamily Member 10Apo-2 LigandApo-2lTnf-Related Apoptosis-Inducing LigandProtein TrailCd Antigen Cd253 |
Database Links | Reactome: R-HSA-140534Reactome: R-HSA-3371378Reactome: R-HSA-5213460Reactome: R-HSA-5218900Reactome: R-HSA-69416Reactome: R-HSA-75158 |
Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinSecretedExists Both As Membrane-Bound And Soluble Form |
Alternative CLIA Names | Tumor Necrosis Factor Ligand Superfamily Member 10 CLIA kitApo-2 Ligand CLIA kitApo-2l CLIA kitTnf-Related Apoptosis-Inducing Ligand CLIA kitProtein Trail CLIA kitCd Antigen Cd253 CLIA kitTNFSF10 CLIA kitAPO2L CLIA kitTRAIL CLIA kit |
Specificity | This kit recognizes Human TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Human TRAIL/TNFSF10 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 93-106 | 100 |
EDTA plasma(n=8) | 93-109 | 100 |
Cell culture media(n=8) | 95-111 | 101 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 25.76 | 79.38 | 247.65 | 25.13 | 83.06 | 237.53 |
Standard deviation | 2.74 | 8.76 | 16.00 | 2.70 | 6.65 | 26.94 |
CV (%) | 10.64 | 11.04 | 6.46 | 10.74 | 8.01 | 11.34 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance