| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human TNFRSF1B kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
TNFRSF1B |
| Gene ID: |
7133 |
| Uniprot ID: |
TNR1B_HUMAN |
| Specificity: |
This kit recognizes Human TNFRSF1B in samples. No significant cross-reactivity or interference between Human TNFRSF1B and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated.mainly on serine residues and with a very low level on threonine residues. A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. |
| Function | Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. |
| Protein Name | Tumor Necrosis Factor Receptor Superfamily Member 1bTumor Necrosis Factor Receptor 2Tnf-R2Tumor Necrosis Factor Receptor Type IiTnf-RiiTnfr-IiP75P80 Tnf-Alpha ReceptorCd Antigen Cd120bEtanercept Cleaved Into - Tumor Necrosis Factor Receptor Superfamily Member 1b - Membrane Form - Tumor Necrosis Factor-Binding Protein 2Tbp-2Tbpii |
| Database Links | Reactome: R-HSA-5668541Reactome: R-HSA-5669034Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: SecretedTumor Necrosis Factor-Binding Protein 2: Secreted |
| Alternative CLIA Names | Tumor Necrosis Factor Receptor Superfamily Member 1b CLIA kitTumor Necrosis Factor Receptor 2 CLIA kitTnf-R2 CLIA kitTumor Necrosis Factor Receptor Type Ii CLIA kitTnf-Rii CLIA kitTnfr-Ii CLIA kitP75 CLIA kitP80 Tnf-Alpha Receptor CLIA kitCd Antigen Cd120b CLIA kitEtanercept Cleaved Into - Tumor Necrosis Factor Receptor Superfamily Member 1b - Membrane Form - Tumor Necrosis Factor-Binding Protein 2 CLIA kitTbp-2 CLIA kitTbpii CLIA kitTNFRSF1B CLIA kitTNFBR CLIA kitTNFR2 CLIA kit |
| Specificity | This kit recognizes Human TNFRSF1B in samples. No significant cross-reactivity or interference between Human TNFRSF1B and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-117 | 108 |
| EDTA plasma(n=8) | 91-107 | 97 |
| Cell culture media(n=8) | 99-115 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 26.80 | 56.79 | 202.70 | 26.24 | 55.21 | 199.08 |
| Standard deviation | 2.32 | 4.83 | 16.72 | 2.51 | 4.42 | 12.96 |
| CV (%) | 8.66 | 8.51 | 8.25 | 9.57 | 8.01 | 6.51 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
