• Human TNFRSF1A (Tumor necrosis factor receptor superfamily member 1A) Sandwich ELISA (STJE0002062)

Human TNFRSF1A (Tumor necrosis factor receptor superfamily member 1A) Sandwich ELISA (STJE0002062)

SKU:
STJE0002062

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This TNFRSF1A Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TNFRSF1A
Gene ID: 7132
Uniprot ID: TNR1A_HUMAN
Specificity: This kit recognizes Human TNFRSF1A in samples. No significant cross-reactivity or interference between Human TNFRSF1A and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 1a
Tumor Necrosis Factor Receptor 1
Tnf-R1
Tumor Necrosis Factor Receptor Type I
Tnf-Ri
Tnfr-I
P55
P60
Cd Antigen Cd120a Cleaved Into - Tumor Necrosis Factor Receptor Superfamily Member 1a - Membrane Form - Tumor Necrosis Factor-Binding Protein 1
Tbpi
Database Links Reactome: R-HSA-5357786
Reactome: R-HSA-5357905
Reactome: R-HSA-5357956
Reactome: R-HSA-5626978
Reactome: R-HSA-5669034
Reactome: R-HSA-6783783
Reactome: R-HSA-75893
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus Membrane
Secreted
A Secreted Form Is Produced Through Proteolytic Processing
Isoform 4: Secreted
Lacks A Golgi-Retention Motif
Is Not Membrane Bound And Therefore Is Secreted
Alternative ELISA Names Tumor Necrosis Factor Receptor Superfamily Member 1a ELISA kit
Tumor Necrosis Factor Receptor 1 ELISA kit
Tnf-R1 ELISA kit
Tumor Necrosis Factor Receptor Type I ELISA kit
Tnf-Ri ELISA kit
Tnfr-I ELISA kit
P55 ELISA kit
P60 ELISA kit
Cd Antigen Cd120a Cleaved Into - Tumor Necrosis Factor Receptor Superfamily Member 1a - Membrane Form - Tumor Necrosis Factor-Binding Protein 1 ELISA kit
Tbpi ELISA kit
TNFRSF1A ELISA kit
TNFAR ELISA kit
TNFR1 ELISA kit
Specificity This kit recognizes Human TNFRSF1A in samples. No significant cross-reactivity or interference between Human TNFRSF1A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-103 95
EDTA plasma (n=8) 94-108 101
Cell culture media (n=8) 92-103 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 23.21 74.91 187.90 20.91 67.85 199.93
Standard deviation 1.27 3.03 7.27 1.40 4.05 10.96
CV (%) 5.47 4.04 3.87 6.70 5.97 5.48
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance