| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This TLR9 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
TLR9 |
| Gene ID: |
54106 |
| Uniprot ID: |
TLR9_HUMAN |
| Specificity: |
This kit recognizes Human TLR9 in samples. No significant cross-reactivity or interference between Human TLR9 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Activated by proteolytic cleavage of the flexible loop between repeats LRR14 and LRR15 within the ectodomain. Cleavage requires UNC93B1. Proteolytically processed by first removing the majority of the ectodomain by either asparagine endopeptidase (AEP) or a cathepsin followed by a trimming event that is solely cathepsin mediated and required for optimal receptor signaling. |
| Function | Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production. |
| Protein Name | Toll-Like Receptor 9Cd Antigen Cd289 |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1679131Reactome: R-HSA-168138Reactome: R-HSA-9679191Reactome: R-HSA-975110Reactome: R-HSA-975138Reactome: R-HSA-975155 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinEndosomeLysosomeCytoplasmic VesiclePhagosomeRelocalizes From Endoplasmic Reticulum To Endosome And Lysosome Upon Stimulation With AgonistExit From The Er Requires Unc93b1Endolysosomal Localization Is Required For Proteolytic Cleavage And Subsequent ActivationIntracellular Localization Of The Active Receptor May Prevent From Responding To Self Nucleic Acid |
| Alternative ELISA Names | Toll-Like Receptor 9 ELISA kitCd Antigen Cd289 ELISA kitTLR9 ELISA kitUNQ5798 ELISA kitPRO19605 ELISA kit |
| Specificity | This kit recognizes Human TLR9 in samples. No significant cross-reactivity or interference between Human TLR9 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-101 | 95 |
| EDTA plasma (n=8) | 90-105 | 97 |
| Cell culture media (n=8) | 87-101 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.49 | 1.23 | 4.38 | 0.51 | 1.24 | 4.15 |
| Standard deviation | 0.03 | 0.07 | 0.20 | 0.03 | 0.06 | 0.18 |
| CV (%) | 6.12 | 5.69 | 4.57 | 5.88 | 4.84 | 4.34 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
