Human TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) Sandwich ELISA (STJE0019877)

SKU:
STJE0019877

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 3.52 pg/mL
Detection Limit: 6.25-400 pg/mL
Short Description: This TIMP-4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TIMP4
Gene ID: 7079
Uniprot ID: TIMP4_HUMAN
Specificity: This kit recognizes Human TIMP-4 in samples. No significant cross-reactivity or interference between Human TIMP-4 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity Abundant in heart and present at low levels in many other tissues.
Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Protein Name Metalloproteinase Inhibitor 4
Tissue Inhibitor Of Metalloproteinases 4
Timp-4
Cellular Localisation Secreted
Alternative ELISA Names Metalloproteinase Inhibitor 4 ELISA kit
Tissue Inhibitor Of Metalloproteinases 4 ELISA kit
Timp-4 ELISA kit
TIMP4 ELISA kit
Specificity This kit recognizes Human TIMP-4 in samples. No significant cross-reactivity or interference between Human TIMP-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-97 92
EDTA plasma (n=8) 85-97 92
Cell culture media (n=8) 85-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 20.90 58.02 191.32 21.91 63.24 199.20
Standard deviation 1.20 3.31 5.78 1.21 2.63 9.28
CV (%) 5.73 5.71 3.02 5.53 4.16 4.66
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance