| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
3.52 pg/mL |
| Detection Limit: |
6.25-400 pg/mL |
| Short Description: |
This TIMP-4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
TIMP4 |
| Gene ID: |
7079 |
| Uniprot ID: |
TIMP4_HUMAN |
| Specificity: |
This kit recognizes Human TIMP-4 in samples. No significant cross-reactivity or interference between Human TIMP-4 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Abundant in heart and present at low levels in many other tissues. |
| Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. |
| Protein Name | Metalloproteinase Inhibitor 4Tissue Inhibitor Of Metalloproteinases 4Timp-4 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Metalloproteinase Inhibitor 4 ELISA kitTissue Inhibitor Of Metalloproteinases 4 ELISA kitTimp-4 ELISA kitTIMP4 ELISA kit |
| Specificity | This kit recognizes Human TIMP-4 in samples. No significant cross-reactivity or interference between Human TIMP-4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-97 | 92 |
| EDTA plasma (n=8) | 85-97 | 92 |
| Cell culture media (n=8) | 85-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 20.90 | 58.02 | 191.32 | 21.91 | 63.24 | 199.20 |
| Standard deviation | 1.20 | 3.31 | 5.78 | 1.21 | 2.63 | 9.28 |
| CV (%) | 5.73 | 5.71 | 3.02 | 5.53 | 4.16 | 4.66 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
