| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This human TGM2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | TGM2 |
| Gene ID: | 7052 |
| Uniprot ID: | TGM2_HUMAN |
| Specificity: | This kit recognizes Human TGM2 in samples. No significant cross-reactivity or interference between Human TGM2 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Disulfide bond formation inactivates the calcium-dependent acyltransferase activity. Cys-370 can form disulfide bonds with both Cys-230 and Cys-371: formation of a disulfide bond between Cys-230 and Cys-370 facilitates formation of the disulfide between Cys-370 and Cys-371, which promotes inactivation of the acyltransferase activity. May also form interchain disulfids between Cys-230 and Cys-370. Ca(2+) protects against disulfide bond formation and inactivation. Auto-transglutaminated: Forms covalent cross-links mediated by transglutaminase between Gln-633 and the epsilon-amino group of a lysine residue of itself or HMGB1, forming homopolymers and heteropolymers, respectively. S-nitrosylated, leading to inactivation of the acyltransferase activity. |
| Function | Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively. Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis. Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54. Under physiological conditions, the protein cross-linking activity is inhibited by GTP.inhibition is relieved by Ca(2+) in response to various stresses. When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds. Plays a key role during apoptosis, both by (1) promoting the cross-linking of cytoskeletal proteins resulting in condensation of the cytoplasm, and by (2) mediating cross-linking proteins of the extracellular matrix, resulting in the irreversible formation of scaffolds that stabilize the integrity of the dying cells before their clearance by phagocytosis, thereby preventing the leakage of harmful intracellular components. In addition to protein cross-linking, can use different monoamine substrates to catalyze a vast array of protein post-translational modifications: mediates aminylation of serotonin, dopamine, noradrenaline or histamine into glutamine residues of target proteins to generate protein serotonylation, dopaminylation, noradrenalinylation or histaminylation, respectively. Mediates protein serotonylation of small GTPases during activation and aggregation of platelets, leading to constitutive activation of these GTPases. Plays a key role in chromatin organization by mediating serotonylation and dopaminylation of histone H3. Catalyzes serotonylation of 'Gln-5' of histone H3 (H3Q5ser) during serotonergic neuron differentiation, thereby facilitating transcription. Acts as a mediator of neurotransmission-independent role of nuclear dopamine in ventral tegmental area (VTA) neurons: catalyzes dopaminylation of 'Gln-5' of histone H3 (H3Q5dop), thereby regulating relapse-related transcriptional plasticity in the reward system. Regulates vein remodeling by mediating serotonylation and subsequent inactivation of ATP2A2/SERCA2. Also acts as a protein deamidase by mediating the side chain deamidation of specific glutamine residues of proteins to glutamate. Catalyzes specific deamidation of protein gliadin, a component of wheat gluten in the diet. May also act as an isopeptidase cleaving the previously formed cross-links. Also able to participate in signaling pathways independently of its acyltransferase activity: acts as a signal transducer in alpha-1 adrenergic receptor-mediated stimulation of phospholipase C-delta (PLCD) activity and is required for coupling alpha-1 adrenergic agonists to the stimulation of phosphoinositide lipid metabolism. Isoform 2: Has cytotoxic activity: is able to induce apoptosis independently of its acyltransferase activity. |
| Protein Name | Protein-Glutamine Gamma-Glutamyltransferase 2Erythrocyte TransglutaminaseHeart G Alpha(HHhg Alpha(HIsopeptidase Tgm2Protein G Alpha(HG(HProtein-Glutamine Deamidase Tgm2Protein-Glutamine Dopaminyltransferase Tgm2Protein-Glutamine Histaminyltransferase Tgm2Protein-Glutamine Noradrenalinyltransferase Tgm2Protein-Glutamine Serotonyltransferase Tgm2Tissue TransglutaminaseTtgTtgaseTransglutaminase CTg(CTgcTgase CTransglutaminase HTgase HTransglutaminase IiTgase IiTransglutaminase-2Tg2Tgase-2Htg2 |
| Cellular Localisation | CytoplasmCytosolNucleusChromosomeSecretedExtracellular SpaceExtracellular MatrixCell MembraneMitochondrionMainly Localizes To The CytosolPresent At Much Lower Level In The Nucleus And ChromatinAlso Secreted Via A Non-Classical Secretion Pathway To The Extracellular MatrixIsoform 2: CytoplasmPerinuclear Region |
| Alternative CLIA Names | Protein-Glutamine Gamma-Glutamyltransferase 2 CLIA kitErythrocyte Transglutaminase CLIA kitHeart G Alpha(H CLIA kitHhg Alpha(H CLIA kitIsopeptidase Tgm2 CLIA kitProtein G Alpha(H CLIA kitG(H CLIA kitProtein-Glutamine Deamidase Tgm2 CLIA kitProtein-Glutamine Dopaminyltransferase Tgm2 CLIA kitProtein-Glutamine Histaminyltransferase Tgm2 CLIA kitProtein-Glutamine Noradrenalinyltransferase Tgm2 CLIA kitProtein-Glutamine Serotonyltransferase Tgm2 CLIA kitTissue Transglutaminase CLIA kitTtg CLIA kitTtgase CLIA kitTransglutaminase C CLIA kitTg(C CLIA kitTgc CLIA kitTgase C CLIA kitTransglutaminase H CLIA kitTgase H CLIA kitTransglutaminase Ii CLIA kitTgase Ii CLIA kitTransglutaminase-2 CLIA kitTg2 CLIA kitTgase-2 CLIA kitHtg2 CLIA kitTGM2 CLIA kit |
| Specificity | This kit recognizes Human TGM2 in samples. No significant cross-reactivity or interference between Human TGM2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-106 | 99 |
| EDTA plasma(n=8) | 97-110 | 104 |
| Cell culture media(n=8) | 93-108 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 93.24 | 199.01 | 925.97 | 100.67 | 188.15 | 1012.30 |
| Standard deviation | 12.06 | 20.42 | 61.95 | 9.80 | 16.03 | 74.40 |
| CV (%) | 12.93 | 10.26 | 6.69 | 9.73 | 8.52 | 7.35 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
