• Human TFR (Transferrin Receptor) Sandwich ELISA Kit (STJE0012494)

Human TFR (Transferrin Receptor) Sandwich ELISA Kit (STJE0012494)

SKU:
STJE0012494

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.58ng/mL
Detection Limit: 1.56-100ng/mL
Short Description: This TFR Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: TFRC
Gene ID: 7037
Uniprot ID: TFR1_HUMAN
Sample Type: serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity
Post Translational Modifications Stearoylated by ZDHHC6 which inhibits TFRC-mediated activation of the JNK pathway and promotes mitochondrial fragmentation. Stearoylation does not affect iron uptake. N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Function Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion. When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2. (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Protein Name Transferrin Receptor Protein 1
Tr
Tfr
Tfr1
Trfr
T9
P90
Cd Antigen Cd71 Cleaved Into - Transferrin Receptor Protein 1 - Serum Form
Stfr
Database Links Reactome: R-HSA-432722
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013406
Reactome: R-HSA-9013407
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Reactome: R-HSA-917977
Reactome: R-HSA-9696270
Reactome: R-HSA-9696273
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Transferrin Receptor Protein 1
Serum Form: Secreted
Alternative ELISA Names Transferrin Receptor Protein 1 ELISA kit
Tr ELISA kit
Tfr ELISA kit
Tfr1 ELISA kit
Trfr ELISA kit
T9 ELISA kit
P90 ELISA kit
Cd Antigen Cd71 Cleaved Into - Transferrin Receptor Protein 1 - Serum Form ELISA kit
Stfr ELISA kit
TFRC ELISA kit
output

Information sourced from Uniprot.org

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