| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88 Mu g/mL |
| Detection Limit: |
3.13~200 Mu g/mL |
| Short Description: |
This TF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
TF |
| Gene ID: |
7018 |
| Uniprot ID: |
TRFE_HUMAN |
| Specificity: |
This kit recognizes Human TF in samples. No significant cross-reactivity or interference between Human TF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6:ESAG7 and extract its iron for its own use. |
| Protein Name | SerotransferrinTransferrinBeta-1 Metal-Binding GlobulinSiderophilin |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-381426Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8957275Reactome: R-HSA-917937Reactome: R-HSA-917977 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Serotransferrin ELISA kitTransferrin ELISA kitBeta-1 Metal-Binding Globulin ELISA kitSiderophilin ELISA kitTF ELISA kitPRO1400 ELISA kit |
| Specificity | This kit recognizes Human TF in samples. No significant cross-reactivity or interference between Human TF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-101 | 97 |
| EDTA plasma (n=8) | 84-93 | 90 |
| Cell culture media (n=8) | 95-106 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (μg/mL) | 8.13 | 23.58 | 80.94 | 8.16 | 21.11 | 86.48 |
| Standard deviation | 0.48 | 1.10 | 3.44 | 0.47 | 1.00 | 4.06 |
| CV (%) | 5.90 | 4.66 | 4.25 | 5.76 | 4.74 | 4.69 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
