• Human TF (Serotransferrin) Sandwich ELISA (STJE0001943)

Human TF (Serotransferrin) Sandwich ELISA (STJE0001943)

SKU:
STJE0001943

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This TF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: F3
Gene ID: 2152
Uniprot ID: TF_HUMAN
Specificity: This kit recognizes Human TF in samples. No significant cross-reactivity or interference between Human TF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The TF:VIIa complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Protein Name Tissue Factor
Tf
Coagulation Factor Iii
Thromboplastin
Cd Antigen Cd142
Database Links Reactome: R-HSA-140834
Reactome: R-HSA-9031628
Cellular Localisation Isoform 1: Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative ELISA Names Tissue Factor ELISA kit
Tf ELISA kit
Coagulation Factor Iii ELISA kit
Thromboplastin ELISA kit
Cd Antigen Cd142 ELISA kit
F3 ELISA kit
Specificity This kit recognizes Human F3/TF in samples. No significant cross-reactivity or interference between Human F3/TF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 92
EDTA plasma (n=8) 92-106 99
Cell culture media (n=8) 92-104 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 27.10 60.96 237.83 27.43 58.54 231.58
Standard deviation 1.80 2.58 8.92 1.62 2.41 11.90
CV (%) 6.64 4.23 3.75 5.91 4.12 5.14
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance