• Human TAGLN (Transgelin) CLIA Kit (STJC0000527)

Human TAGLN (Transgelin) CLIA Kit (STJC0000527)

SKU:
STJC0000527

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.75ng/mL
Detection Limit: 1.25~80ng/mL
Short Description: This human TAGLN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: TAGLN
Gene ID: 6876
Uniprot ID: TAGL_HUMAN
Specificity: This kit recognizes Human TAGLN in samples. No significant cross-reactivity or interference between Human TAGLN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Actin cross-linking/gelling protein. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
Protein Name Transgelin
22 Kda Actin-Binding Protein
Protein Ws3-10
Smooth Muscle Protein 22-Alpha
Sm22-Alpha
Cellular Localisation Cytoplasm
Alternative CLIA Names Transgelin CLIA kit
22 Kda Actin-Binding Protein CLIA kit
Protein Ws3-10 CLIA kit
Smooth Muscle Protein 22-Alpha CLIA kit
Sm22-Alpha CLIA kit
TAGLN CLIA kit
SM22 CLIA kit
WS3-10 CLIA kit
Specificity This kit recognizes Human TAGLN in samples. No significant cross-reactivity or interference between Human TAGLN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-103 97
EDTA plasma(n=8) 99-111 105
Cell culture media(n=8) 99-115 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 4.21 11.25 28.58 4.00 10.73 30.86
Standard deviation 0.52 0.89 2.08 0.46 0.83 2.37
CV (%) 12.35 7.91 7.28 11.50 7.74 7.68
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance