| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This STUB1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
STUB1 |
| Gene ID: |
10273 |
| Uniprot ID: |
CHIP_HUMAN |
| Specificity: |
This kit recognizes Human STUB1 in samples. No significant cross-reactivity or interference between Human STUB1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Monoubiquitinated at Lys-2 following cell stress by UBE2W, promoting the interaction with ATXN3. Auto-ubiquitinated.mediated by UBE2D1 and UBE2D2. |
| Function | E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Acts as a co-chaperone for HSPA1A and HSPA1B chaperone proteins and promotes ubiquitin-mediated protein degradation. Negatively regulates the suppressive function of regulatory T-cells (Treg) during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner. Catalyzes monoubiquitination of SIRT6, preventing its degradation by the proteasome. Likely mediates polyubiquitination and down-regulates plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Negatively regulates TGF-beta signaling by modulating the basal level of SMAD3 via ubiquitin-mediated degradation. May regulate myosin assembly in striated muscles together with UBE4B and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation. Mediates ubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation. |
| Protein Name | E3 Ubiquitin-Protein Ligase ChipAntigen Ny-Co-7Cll-Associated Antigen Kw-8Carboxy Terminus Of Hsp70-Interacting ProteinRing-Type E3 Ubiquitin Transferase ChipStip1 Homology And U Box-Containing Protein 1 |
| Database Links | Reactome: R-HSA-2173788Reactome: R-HSA-5213460Reactome: R-HSA-5357905Reactome: R-HSA-5675482Reactome: R-HSA-8863795Reactome: R-HSA-8939902Reactome: R-HSA-8948751Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusTranslocates To The Nucleus In Response To Inflammatory Signals In Regulatory T-Cells (Treg) |
| Alternative ELISA Names | E3 Ubiquitin-Protein Ligase Chip ELISA kitAntigen Ny-Co-7 ELISA kitCll-Associated Antigen Kw-8 ELISA kitCarboxy Terminus Of Hsp70-Interacting Protein ELISA kitRing-Type E3 Ubiquitin Transferase Chip ELISA kitStip1 Homology And U Box-Containing Protein 1 ELISA kitSTUB1 ELISA kitCHIP ELISA kitPP1131 ELISA kit |
| Specificity | This kit recognizes Human STUB1 in samples. No significant cross-reactivity or interference between Human STUB1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-108 | 98 |
| EDTA plasma (n=8) | 92-106 | 99 |
| Cell culture media (n=8) | 91-104 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 94.62 | 307.91 | 875.56 | 102.66 | 293.71 | 958.48 |
| Standard deviation | 6.34 | 12.69 | 33.36 | 5.40 | 15.10 | 52.43 |
| CV (%) | 6.70 | 4.12 | 3.81 | 5.26 | 5.14 | 5.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
