• Human SRSF2 (Serine/Arginine Rich Splicing Factor 2) Sandwich ELISA Kit Ready-To-Use (STJE0018728)

Human SRSF2 (Serine/Arginine Rich Splicing Factor 2) Sandwich ELISA Kit Ready-To-Use (STJE0018728)

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STJE0018728

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.054ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This SRSF2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: SRSF2
Gene ID: 6427
Uniprot ID: SRSF2_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity
Post Translational Modifications Extensively phosphorylated on serine residues in the RS domain. Phosphorylated by SRPK2 and this causes its redistribution from the nuclear speckle to nucleoplasm and controls cell fate decision in response to cisplatin treatment. KAT5/TIP60 inhibits its phosphorylation by preventing SRPK2 nuclear translocation. Acetylation on Lys-52 by KAT5/TIP60 promotes its proteasomal degradation. This effect is counterbalanced by HDAC6, which positively controls SRSF2 protein level by deacetylating it and preventing its proteasomal degradation.
Function Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.
Protein Name Serine/Arginine-Rich Splicing Factor 2
Protein Pr264
Splicing Component - 35 Kda
Splicing Factor Sc35
Sc-35
Splicing Factor - Arginine/Serine-Rich 2
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Cellular Localisation Nucleus
Nucleoplasm
Nucleus Speckle
Phosphorylation By Srpk2 Provokes Its Redistribution From The Nuclear Speckle To Nucleoplasm
Alternative ELISA Names Serine/Arginine-Rich Splicing Factor 2 ELISA kit
Protein Pr264 ELISA kit
Splicing Component - 35 Kda ELISA kit
Splicing Factor Sc35 ELISA kit
Sc-35 ELISA kit
Splicing Factor - Arginine/Serine-Rich 2 ELISA kit
SRSF2 ELISA kit
SFRS2 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance