• Human SOST (Sclerostin) Sandwich ELISA (STJE0003047)

Human SOST (Sclerostin) Sandwich ELISA (STJE0003047)

SKU:
STJE0003047

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This SOST Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: SOST
Gene ID: 50964
Uniprot ID: SOST_HUMAN
Specificity: This kit recognizes Human SOST in samples. No significant cross-reactivity or interference between Human SOST and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Protein Name Sclerostin
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3772470
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative ELISA Names Sclerostin ELISA kit
SOST ELISA kit
UNQ2976 ELISA kit
PRO7455 ELISA kit
PRO7476 ELISA kit
Specificity This kit recognizes Human SOST in samples. No significant cross-reactivity or interference between Human SOST and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 91
EDTA plasma (n=8) 92-107 98
Cell culture media (n=8) 88-103 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 222.11 370.69 1524.64 203.00 389.31 1557.60
Standard deviation 11.82 15.05 67.24 11.27 21.30 67.60
CV (%) 5.32 4.06 4.41 5.55 5.47 4.34