Applications: | ELISA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 37.5pg/mL |
Detection Limit: | 62.5~4000pg/mL |
Short Description: | This SOST Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | SOST |
Gene ID: | 50964 |
Uniprot ID: | SOST_HUMAN |
Specificity: | This kit recognizes Human SOST in samples. No significant cross-reactivity or interference between Human SOST and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. |
Protein Name | Sclerostin |
Database Links | Reactome: R-HSA-201681 Reactome: R-HSA-3772470 |
Cellular Localisation | Secreted Extracellular Space Extracellular Matrix |
Alternative ELISA Names | Sclerostin ELISA kit SOST ELISA kit UNQ2976 ELISA kit PRO7455 ELISA kit PRO7476 ELISA kit |
Specificity | This kit recognizes Human SOST in samples. No significant cross-reactivity or interference between Human SOST and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 86-99 | 91 |
EDTA plasma (n=8) | 92-107 | 98 |
Cell culture media (n=8) | 88-103 | 94 |
Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
---|---|---|---|---|---|---|
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 222.11 | 370.69 | 1524.64 | 203.00 | 389.31 | 1557.60 |
Standard deviation | 11.82 | 15.05 | 67.24 | 11.27 | 21.30 | 67.60 |
CV (%) | 5.32 | 4.06 | 4.41 | 5.55 | 5.47 | 4.34 |