| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This SMAD7 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SMAD7 |
| Gene ID: |
4092 |
| Uniprot ID: |
SMAD7_HUMAN |
| Specificity: |
This kit recognizes Human Smad7/MADH7 in samples. No significant cross-reactivity or interference between Human Smad7/MADH7 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling.however it affects its ability to regulate transcription. Phosphorylated by PDPK1. Ubiquitinated by WWP1. Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1.which promotes its degradation. Acetylation prevents ubiquitination and degradation mediated by SMURF1. |
| Function | Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members.has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Protein Name | Mothers Against Decapentaplegic Homolog 7Mad Homolog 7Mothers Against Dpp Homolog 7Mothers Against Decapentaplegic Homolog 8Mad Homolog 8Mothers Against Dpp Homolog 8Smad Family Member 7Smad 7Smad7Hsmad7 |
| Database Links | Reactome: R-HSA-201451Reactome: R-HSA-2173788Reactome: R-HSA-2173795Reactome: R-HSA-2173796Reactome: R-HSA-5689603Reactome: R-HSA-5689880Reactome: R-HSA-877300 |
| Cellular Localisation | NucleusCytoplasmInteraction With Nedd4l Or Rnf111 Induces Translocation From The Nucleus To The CytoplasmTgf-Beta Stimulates Its Translocation From The Nucleus To The CytoplasmPdpk1 Inhibits Its Translocation From The Nucleus To The Cytoplasm In Response To Tgf-Beta |
| Alternative ELISA Names | Mothers Against Decapentaplegic Homolog 7 ELISA kitMad Homolog 7 ELISA kitMothers Against Dpp Homolog 7 ELISA kitMothers Against Decapentaplegic Homolog 8 ELISA kitMad Homolog 8 ELISA kitMothers Against Dpp Homolog 8 ELISA kitSmad Family Member 7 ELISA kitSmad 7 ELISA kitSmad7 ELISA kitHsmad7 ELISA kitSMAD7 ELISA kitMADH7 ELISA kitMADH8 ELISA kit |
| Specificity | This kit recognizes Human Smad7/MADH7 in samples. No significant cross-reactivity or interference between Human Smad7/MADH7 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-109 | 101 |
| EDTA plasma (n=8) | 86-98 | 91 |
| Cell culture media (n=8) | 91-104 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.52 | 1.04 | 3.81 | 0.49 | 1.02 | 3.95 |
| Standard deviation | 0.03 | 0.06 | 0.16 | 0.03 | 0.05 | 0.16 |
| CV (%) | 5.77 | 5.77 | 4.20 | 6.12 | 4.90 | 4.05 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
