• Human SKP2 (S-Phase Kinase Associated Protein 2) Sandwich ELISA Kit (STJE0012183)

Human SKP2 (S-Phase Kinase Associated Protein 2) Sandwich ELISA Kit (STJE0012183)

SKU:
STJE0012183

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.061ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This SKP2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: SKP2
Gene ID: 6502
Uniprot ID: SKP2_HUMAN
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity
Post Translational Modifications Ubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Deubiquitinated by USP13. Acetylation at Lys-68 and Lys-71 increases stability through impairment of APC/C-mediated proteolysis and promotes cytoplasmic retention. Deacetylated by SIRT3.
Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration. Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus.
Protein Name S-Phase Kinase-Associated Protein 2
Cyclin-A/Cdk2-Associated Protein P45
F-Box Protein Skp2
F-Box/Lrr-Repeat Protein 1
P45skp2
Database Links Reactome: R-HSA-174178
Reactome: R-HSA-187577
Reactome: R-HSA-5689880
Reactome: R-HSA-68949
Reactome: R-HSA-69231
Reactome: R-HSA-8939902
Reactome: R-HSA-8951664
Reactome: R-HSA-9687136
Reactome: R-HSA-9708530
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Alternative ELISA Names S-Phase Kinase-Associated Protein 2 ELISA kit
Cyclin-A/Cdk2-Associated Protein P45 ELISA kit
F-Box Protein Skp2 ELISA kit
F-Box/Lrr-Repeat Protein 1 ELISA kit
P45skp2 ELISA kit
SKP2 ELISA kit
FBXL1 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance