| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.061ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This SKP2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
SKP2 |
| Gene ID: |
6502 |
| Uniprot ID: |
SKP2_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Ubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Deubiquitinated by USP13. Acetylation at Lys-68 and Lys-71 increases stability through impairment of APC/C-mediated proteolysis and promotes cytoplasmic retention. Deacetylated by SIRT3. |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration. Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus. |
| Protein Name | S-Phase Kinase-Associated Protein 2Cyclin-A/Cdk2-Associated Protein P45F-Box Protein Skp2F-Box/Lrr-Repeat Protein 1P45skp2 |
| Database Links | Reactome: R-HSA-174178Reactome: R-HSA-187577Reactome: R-HSA-5689880Reactome: R-HSA-68949Reactome: R-HSA-69231Reactome: R-HSA-8939902Reactome: R-HSA-8951664Reactome: R-HSA-9687136Reactome: R-HSA-9708530Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative ELISA Names | S-Phase Kinase-Associated Protein 2 ELISA kitCyclin-A/Cdk2-Associated Protein P45 ELISA kitF-Box Protein Skp2 ELISA kitF-Box/Lrr-Repeat Protein 1 ELISA kitP45skp2 ELISA kitSKP2 ELISA kitFBXL1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
