| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.124ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This SIRT3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
SIRT3 |
| Gene ID: |
23410 |
| Uniprot ID: |
SIR3_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. |
| Function | NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation. Regulates hepatic lipogenesis. Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. |
| Protein Name | Nad-Dependent Protein Deacetylase Sirtuin-3 - MitochondrialHsirt3Regulatory Protein Sir2 Homolog 3Sir2-Like Protein 3 |
| Database Links | Reactome: R-HSA-2151201Reactome: R-HSA-9615017Reactome: R-HSA-9617629 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative ELISA Names | Nad-Dependent Protein Deacetylase Sirtuin-3 - Mitochondrial ELISA kitHsirt3 ELISA kitRegulatory Protein Sir2 Homolog 3 ELISA kitSir2-Like Protein 3 ELISA kitSIRT3 ELISA kitSIR2L3 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
