| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.28 fmol/mL |
| Detection Limit: |
7.81-500 fmol/mL |
| Short Description: |
This SHBG Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SHBG |
| Gene ID: |
6462 |
| Uniprot ID: |
SHBG_HUMAN |
| Specificity: |
This kit recognizes Human SHBG in samples. No significant cross-reactivity or interference between Human SHBG and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Isoform 1 and isoform 2 are present in liver and testis. |
| Post Translational Modifications | Variant Asn-356 contains one N-linked (GlcNAc..) at position 356. |
| Function | Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. |
| Protein Name | Sex Hormone-Binding GlobulinShbgSex Steroid-Binding ProteinSbpTestis-Specific Androgen-Binding ProteinAbpTestosterone-Estradiol-Binding GlobulinTebgTestosterone-Estrogen-Binding Globulin |
| Cellular Localisation | SecretedIn TestisIt Is Synthesized By The Sertoli CellsSecreted Into The Lumen Of The Seminiferous Tubule And Transported To The Epididymis |
| Alternative ELISA Names | Sex Hormone-Binding Globulin ELISA kitShbg ELISA kitSex Steroid-Binding Protein ELISA kitSbp ELISA kitTestis-Specific Androgen-Binding Protein ELISA kitAbp ELISA kitTestosterone-Estradiol-Binding Globulin ELISA kitTebg ELISA kitTestosterone-Estrogen-Binding Globulin ELISA kitSHBG ELISA kit |
| Specificity | This kit recognizes Human SHBG in samples. No significant cross-reactivity or interference between Human SHBG and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-101 | 94 |
| EDTA plasma (n=8) | 94-106 | 101 |
| Cell culture media (n=8) | 84-98 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (fmol/mL) | 27.07 | 53.05 | 181.43 | 26.49 | 52.67 | 187.50 |
| Standard deviation | 1.52 | 2.98 | 6.95 | 1.84 | 3.00 | 7.44 |
| CV (%) | 5.61 | 5.62 | 3.83 | 6.96 | 5.70 | 3.97 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
