• Human SFTPB (Pulmonary surfactant-associated protein B) Sandwich ELISA (STJE0002838)
  • Human SFTPB (Pulmonary surfactant-associated protein B) Sandwich ELISA (STJE0002838)

Human SFTPB (Pulmonary surfactant-associated protein B) Sandwich ELISA (STJE0002838)

SKU:
STJE0002838

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This SFTPB Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: SFTPB
Gene ID: 6439
Uniprot ID: PSPB_HUMAN
Specificity: This kit recognizes Human SP-B in samples. No significant cross-reactivity or interference between Human SP-B and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Pulmonary Surfactant-Associated Protein B
Sp-B
18 Kda Pulmonary-Surfactant Protein
6 Kda Protein
Pulmonary Surfactant-Associated Proteolipid Spl(Phe
Database Links Reactome: R-HSA-5683826
Reactome: R-HSA-5688031
Reactome: R-HSA-5688849
Reactome: R-HSA-5688890
Cellular Localisation Secreted
Extracellular Space
Surface Film
Alternative ELISA Names Pulmonary Surfactant-Associated Protein B ELISA kit
Sp-B ELISA kit
18 Kda Pulmonary-Surfactant Protein ELISA kit
6 Kda Protein ELISA kit
Pulmonary Surfactant-Associated Proteolipid Spl(Phe ELISA kit
SFTPB ELISA kit
SFTP3 ELISA kit
Specificity This kit recognizes Human SP-B in samples. No significant cross-reactivity or interference between Human SP-B and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-105 99
EDTA plasma (n=8) 88-99 94
Cell culture media (n=8) 89-101 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.42 7.23 22.89 2.26 7.33 20.64
Standard deviation 0.16 0.35 0.71 0.12 0.41 1.05
CV (%) 6.61 4.84 3.10 5.31 5.59 5.09
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance