| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5ng/mL |
| Detection Limit: |
12.5~800ng/mL |
| Short Description: |
This SERPINF2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SERPINF2 |
| Gene ID: |
5345 |
| Uniprot ID: |
A2AP_HUMAN |
| Specificity: |
This kit recognizes Human Alpha 2-AP in samples. No significant cross-reactivity or interference between Human Alpha 2-AP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Proteolytically cleaved at Pro-39 by both the prolyl endopeptidase FAP form and antiplasmin-cleaving enzyme FAP soluble form to generate mature alpha-2-antiplasmin. |
| Function | Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. |
| Protein Name | Alpha-2-AntiplasminAlpha-2-ApAlpha-2-Plasmin InhibitorAlpha-2-PiSerpin F2 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-75205 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Alpha-2-Antiplasmin ELISA kitAlpha-2-Ap ELISA kitAlpha-2-Plasmin Inhibitor ELISA kitAlpha-2-Pi ELISA kitSerpin F2 ELISA kitSERPINF2 ELISA kitAAP ELISA kitPLI ELISA kit |
| Specificity | This kit recognizes Human α2-AP in samples. No significant cross-reactivity or interference between Human α2-AP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-102 | 97 |
| EDTA plasma (n=8) | 89-102 | 94 |
| Cell culture media (n=8) | 91-107 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 40.88 | 118.96 | 393.16 | 39.63 | 122.85 | 396.21 |
| Standard deviation | 2.07 | 5.92 | 16.00 | 2.22 | 6.15 | 13.87 |
| CV (%) | 5.06 | 4.98 | 4.07 | 5.60 | 5.01 | 3.50 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
