| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human SERPINB3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
SERPINB3 |
| Gene ID: |
6317 |
| Uniprot ID: |
SPB3_HUMAN |
| Specificity: |
This kit recognizes Human SCCA1 in samples. No significant cross-reactivity or interference between Human SCCA1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Squamous cells. Expressed in some hepatocellular carcinoma (at protein level). |
| Function | May act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1). |
| Protein Name | Serpin B3Protein T4-ASquamous Cell Carcinoma Antigen 1Scca-1 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | CytoplasmSeems To Also Be Secreted In Plasma By Cancerous Cells But At A Low Level |
| Alternative CLIA Names | Serpin B3 CLIA kitProtein T4-A CLIA kitSquamous Cell Carcinoma Antigen 1 CLIA kitScca-1 CLIA kitSERPINB3 CLIA kitSCCA CLIA kitSCCA1 CLIA kit |
| Specificity | This kit recognizes Human SCCA1 in samples. No significant cross-reactivity or interference between Human SCCA1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-112 | 102 |
| EDTA plasma(n=8) | 86-98 | 93 |
| Cell culture media(n=8) | 91-103 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 47.27 | 109.41 | 423.38 | 47.67 | 101.57 | 458.79 |
| Standard deviation | 5.65 | 10.89 | 41.11 | 4.60 | 7.94 | 43.26 |
| CV (%) | 11.95 | 9.95 | 9.71 | 9.65 | 7.82 | 9.43 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
