Human Serpin A1/Alpha-1-antitrypsin protein (Recombinant) (C-His) (STJP002614)

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STJP002614
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Host: HEK293 cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Formulation: Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method: < 1.0 EU/Mu g of the protein by LAL method.
Immunoreactivity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 value is approximately <0.4 nM.
Gene Symbol: SERPINA1
Gene ID: 5265
Uniprot ID: A1AT_HUMAN
Immunogen Region: Glu25-Lys418
Immunogen: Recombinant Human Serpin A1/Alpha-1-antitrypsin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu25-Lys418) of human alpha 1 Antitrypsin (Accession #NP_000286.3) fused with a 6×His tag at the C-
Immunogen Sequence: EDPQGDAAQKTDTSHHDQDH PTFNKITPNLAEFAFSLYRQ LAHQSNSTNIFFSPVSIATA FAMLSLGTKADTHDEILEGL NFNLTEIPEAQIHEGFQELL RTLNQPDSQLQLTTGNGLFL SEGLKLVDKFLEDVKKLYHS EAFTVNFGDTEEAKKQINDY VEKGTQGKIVDLVKELDRDT VFALVNYIFFKGKWERPFEV KDTEEEDFHVDQVTTVKVPM MKRLGMFNIQHCKKLSSWV
Post Translational Modifications N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)Man(alpha1-3)Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases.
Function Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Protein Name Alpha-1-Antitrypsin
Alpha-1 Protease Inhibitor
Alpha-1-Antiproteinase
Serpin A1 Cleaved Into - Short Peptide From Aat
Spaat
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-204005
Reactome: R-HSA-381426
Reactome: R-HSA-5694530
Reactome: R-HSA-6798695
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Endoplasmic Reticulum
The S And Z Allele Are Not Secreted Effectively And Accumulate Intracellularly In The Endoplasmic Reticulum
Short Peptide From Aat: Secreted
Extracellular Space
Extracellular Matrix
Alternative Protein Names Alpha-1-Antitrypsin protein
Alpha-1 Protease Inhibitor protein
Alpha-1-Antiproteinase protein
Serpin A1 Cleaved Into - Short Peptide From Aat protein
Spaat protein
SERPINA1 protein
AAT protein
PI protein
PRO0684 protein
PRO2209 protein

Information sourced from Uniprot.org

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