| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This SDCBP2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SDCBP2 |
| Gene ID: |
27111 |
| Uniprot ID: |
SDCB2_HUMAN |
| Specificity: |
This kit recognizes Human ST2 in samples. No significant cross-reactivity or interference between Human ST2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Binds phosphatidylinositol 4,5-bisphosphate (PIP2). May play a role in the organization of nuclear PIP2, cell division and cell survival. |
| Protein Name | Syntenin-2Similar To Tacip18SitacSyndecan-Binding Protein 2 |
| Cellular Localisation | CytoplasmNucleusNucleolusNucleoplasmCell MembraneNucleus SpeckleAssociates With Intracellular Membranes And Enriched In The Apical Region Of The Cell And In Intracellular CompartmentsColocalizes With Tm4sf1 In The Apical Region Of The CellPredominantly Targeted To Nuclear Pip2 PoolsShuttles Between Several Subcellular CompartmentsPip2 Plays An Important Role In The Distribution Of Sdcbp2 |
| Alternative ELISA Names | Syntenin-2 ELISA kitSimilar To Tacip18 ELISA kitSitac ELISA kitSyndecan-Binding Protein 2 ELISA kitSDCBP2 ELISA kitSITAC18 ELISA kit |
| Specificity | This kit recognizes Human ST2 in samples. No significant cross-reactivity or interference between Human ST2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-102 | 96 |
| EDTA plasma (n=8) | 84-97 | 90 |
| Cell culture media (n=8) | 88-100 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 1.20 | 3.58 | 0.45 | 1.31 | 3.80 |
| Standard deviation | 0.03 | 0.07 | 0.18 | 0.02 | 0.06 | 0.18 |
| CV (%) | 6.00 | 5.83 | 5.03 | 4.44 | 4.58 | 4.74 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
