| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.067ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This SCNN1a Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
SCNN1A |
| Gene ID: |
6337 |
| Uniprot ID: |
SCNNA_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in the female reproductive tract, from the fimbrial end of the fallopian tube to the endometrium (at protein level). Expressed in kidney (at protein level). In the respiratory tract, expressed in the bronchial epithelium (at protein level). Highly expressed in lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. in skin, expressed in keratinocytes, melanocytes and Merkel cells of the epidermal sub-layers, stratum basale, stratum spinosum and stratum granulosum (at protein level). Expressed in the outer root sheath of the hair follicles (at protein level). Detected in both peripheral and central cells of the sebaceous gland (at protein level). Expressed by eccrine sweat glands (at protein level). In skin, also expressed by arrector pili muscle cells and intradermal adipocytes. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. |
| Post Translational Modifications | Ubiquitinated.this targets individual subunits for endocytosis and proteasome-mediated degradation. ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation. N-glycosylated. |
| Function | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception. |
| Protein Name | Amiloride-Sensitive Sodium Channel Subunit AlphaAlpha-NachEpithelial Na(+ Channel Subunit AlphaAlpha-EnacEnacaNonvoltage-Gated Sodium Channel 1 Subunit AlphaScnea |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-9730628 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinCell ProjectionCiliumCytoplasmic GranuleCytoplasmCytoplasmic VesicleSecretory VesicleAcrosomeFlagellumIn The Oviduct And BronchusLocated On Cilia In Multi-Ciliated CellsIn Endometrial Non-Ciliated Epithelial CellsRestricted To Apical SurfacesIn EpidermisLocated Nearly Uniformly In The Cytoplasm In A Granular DistributionIn Sebaceous GlandsObserved Only In The Cytoplasmic Space In Between The Lipid VesiclesIn Eccrine Sweat GlandsMainly Located At The Apical Surface Of The Cells Facing The LumenIn SkinIn Arrector Pili Muscle Cells And In AdipocytesLocated In The Cytoplasm And Colocalized With Actin FibersIn SpermatogoniaSpermatocytes And Round SpermatidsLocated In The CytoplasmPrior To SpermiationLocation Shifts From The Cytoplasm To The Spermatid TailIn SpermatozoaLocalizes At The Acrosome And The Central Region Of The Sperm Flagellum |
| Alternative ELISA Names | Amiloride-Sensitive Sodium Channel Subunit Alpha ELISA kitAlpha-Nach ELISA kitEpithelial Na(+ Channel Subunit Alpha ELISA kitAlpha-Enac ELISA kitEnaca ELISA kitNonvoltage-Gated Sodium Channel 1 Subunit Alpha ELISA kitScnea ELISA kitSCNN1A ELISA kitSCNN1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
