| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.75ng/mL |
| Detection Limit: |
1.25~80ng/mL |
| Short Description: |
This sCD14 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CD14 |
| Gene ID: |
929 |
| Uniprot ID: |
CD14_HUMAN |
| Specificity: |
This kit recognizes Human sCD14 in samples. No significant cross-reactivity or interference between Human sCD14 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
| Function | Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). |
| Protein Name | Monocyte Differentiation Antigen Cd14Myeloid Cell-Specific Leucine-Rich GlycoproteinCd Antigen Cd14 Cleaved Into - Monocyte Differentiation Antigen Cd14 - Urinary Form - Monocyte Differentiation Antigen Cd14 - Membrane-Bound Form |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-140534Reactome: R-HSA-166016Reactome: R-HSA-166020Reactome: R-HSA-166058Reactome: R-HSA-166166Reactome: R-HSA-168179Reactome: R-HSA-168188Reactome: R-HSA-2562578Reactome: R-HSA-5602498Reactome: R-HSA-5603041Reactome: R-HSA-5686938Reactome: R-HSA-6798695Reactome: R-HSA-936964Reactome: R-HSA-937041Reactome: R-HSA-937072Reactome: R-HSA-975163 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorSecretedMembrane RaftGolgi ApparatusSecreted Forms May Arise By Cleavage Of The Gpi Anchor |
| Alternative ELISA Names | Monocyte Differentiation Antigen Cd14 ELISA kitMyeloid Cell-Specific Leucine-Rich Glycoprotein ELISA kitCd Antigen Cd14 Cleaved Into - Monocyte Differentiation Antigen Cd14 - Urinary Form - Monocyte Differentiation Antigen Cd14 - Membrane-Bound Form ELISA kitCD14 ELISA kit |
| Specificity | This kit recognizes Human sCD14 in samples. No significant cross-reactivity or interference between Human sCD14 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-100 | 96 |
| EDTA plasma (n=8) | 86-100 | 94 |
| Cell culture media (n=8) | 85-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 3.30 | 8.64 | 39.05 | 3.34 | 8.89 | 37.01 |
| Standard deviation | 0.17 | 0.40 | 2.02 | 0.18 | 0.41 | 1.55 |
| CV (%) | 5.08 | 4.59 | 5.18 | 5.26 | 4.60 | 4.18 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
