| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human S100A6 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
S100A6 |
| Gene ID: |
6277 |
| Uniprot ID: |
S10A6_HUMAN |
| Specificity: |
This kit recognizes Human S100A6 in samples. No significant cross-reactivity or interference between Human S100A6 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative. |
| Protein Name | Protein S100-A6CalcyclinGrowth Factor-Inducible Protein 2a9Mln 4Prolactin Receptor-Associated ProteinPraS100 Calcium-Binding Protein A6 |
| Cellular Localisation | Nucleus EnvelopeCytoplasmCell MembranePeripheral Membrane ProteinCytoplasmic Side |
| Alternative CLIA Names | Protein S100-A6 CLIA kitCalcyclin CLIA kitGrowth Factor-Inducible Protein 2a9 CLIA kitMln 4 CLIA kitProlactin Receptor-Associated Protein CLIA kitPra CLIA kitS100 Calcium-Binding Protein A6 CLIA kitS100A6 CLIA kitCACY CLIA kit |
| Specificity | This kit recognizes Human S100A6 in samples. No significant cross-reactivity or interference between Human S100A6 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 97-109 | 102 |
| EDTA plasma(n=8) | 87-102 | 93 |
| Cell culture media(n=8) | 100-116 | 107 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.65 | 94.70 | 491.62 | 49.55 | 99.18 | 521.23 |
| Standard deviation | 4.97 | 9.87 | 41.44 | 5.14 | 7.61 | 46.65 |
| CV (%) | 9.62 | 10.42 | 8.43 | 10.37 | 7.67 | 8.95 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
