| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.63ng/mL |
| Detection Limit: |
1.56-100ng/mL |
| Short Description: |
This RPN1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
RPN1 |
| Gene ID: |
6184 |
| Uniprot ID: |
RPN1_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in all tissues tested. |
| Post Translational Modifications | Ufmylated by UFL1 in response to endoplasmic reticulum stress, promoting reticulophagy of endoplasmic reticulum sheets. |
| Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. |
| Protein Name | Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda SubunitRibophorin IRpn-IRibophorin-1 |
| Database Links | Reactome: R-HSA-1799339Reactome: R-HSA-446203Reactome: R-HSA-9694548 |
| Cellular Localisation | Endoplasmic ReticulumEndoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative ELISA Names | Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1 ELISA kitDolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda Subunit ELISA kitRibophorin I ELISA kitRpn-I ELISA kitRibophorin-1 ELISA kitRPN1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
