• Human RNASE3 (Eosinophil cationic protein) Sandwich ELISA (STJE0002927)
  • Human RNASE3 (Eosinophil cationic protein) Sandwich ELISA (STJE0002927)

Human RNASE3 (Eosinophil cationic protein) Sandwich ELISA (STJE0002927)

SKU:
STJE0002927

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 23.44pg/mL
Detection Limit: 39.06~2500pg/mL
Short Description: This RNASE3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: RNASE3
Gene ID: 6037
Uniprot ID: ECP_HUMAN
Specificity: This kit recognizes Human RNASE3/ECP in samples. No significant cross-reactivity or interference between Human RNASE3/ECP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Eosinophil Cationic Protein
Ecp
Ribonuclease 3
Rnase 3
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-6803157
Cellular Localisation Secreted
Located In The Matrix Of Eosinophil Large Specific Granule
Which Are Released Following Activation By An Immune Stimulus
Alternative ELISA Names Eosinophil Cationic Protein ELISA kit
Ecp ELISA kit
Ribonuclease 3 ELISA kit
Rnase 3 ELISA kit
RNASE3 ELISA kit
ECP ELISA kit
RNS3 ELISA kit
Specificity This kit recognizes Human RNASE3/ECP in samples. No significant cross-reactivity or interference between Human RNASE3/ECP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-111 101
EDTA plasma (n=8) 95-109 100
Cell culture media (n=8) 92-107 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 123.30 241.53 1242.96 135.11 256.43 1291.01
Standard deviation 6.55 13.21 64.76 9.09 12.87 52.54
CV (%) 5.31 5.47 5.21 6.73 5.02 4.07
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance