• Human RLN2 (Prorelaxin H2) Sandwich ELISA (STJE0003079)
  • Human RLN2 (Prorelaxin H2) Sandwich ELISA (STJE0003079)

Human RLN2 (Prorelaxin H2) Sandwich ELISA (STJE0003079)

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STJE0003079

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This RLN2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: RLN2
Gene ID: 6019
Uniprot ID: REL2_HUMAN
Specificity: This kit recognizes Human RLN2 in samples. No significant cross-reactivity or interference between Human RLN2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.
Protein Name Prorelaxin H2 Cleaved Into - Relaxin B Chain - Relaxin A Chain
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-444821
Cellular Localisation Secreted
Alternative ELISA Names Prorelaxin H2 Cleaved Into - Relaxin B Chain - Relaxin A Chain ELISA kit
RLN2 ELISA kit
Specificity This kit recognizes Human RLN2 in samples. No significant cross-reactivity or interference between Human RLN2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 94
EDTA plasma (n=8) 91-105 96
Cell culture media (n=8) 89-106 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 25.40 43.71 244.95 27.29 41.78 265.59
Standard deviation 1.61 2.57 9.46 1.54 2.34 14.24
CV (%) 6.34 5.88 3.86 5.64 5.60 5.36
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance