| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
3.75pg/mL |
| Detection Limit: |
6.25~400pg/mL |
| Short Description: |
This human RLN2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
RLN2 |
| Gene ID: |
6019 |
| Uniprot ID: |
REL2_HUMAN |
| Specificity: |
This kit recognizes Human RLN2 in samples. No significant cross-reactivity or interference between Human RLN2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Isoform 1 is expressed in the ovary during pregnancy. Also expressed in placenta, decidua and prostate. Isoform 2 is relatively abundant in placenta. It is in much lower abundance in the prostate gland. Not detected in the ovary. |
| Function | Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. |
| Protein Name | Prorelaxin H2 Cleaved Into - Relaxin B Chain - Relaxin A Chain |
| Database Links | Reactome: R-HSA-418555Reactome: R-HSA-444821 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Prorelaxin H2 Cleaved Into - Relaxin B Chain - Relaxin A Chain CLIA kitRLN2 CLIA kit |
| Specificity | This kit recognizes Human RLN2 in samples. No significant cross-reactivity or interference between Human RLN2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 97-111 | 102 |
| EDTA plasma(n=8) | 87-100 | 93 |
| Cell culture media(n=8) | 88-101 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 21.14 | 52.67 | 171.08 | 22.02 | 53.32 | 159.78 |
| Standard deviation | 2.35 | 4.69 | 18.77 | 2.42 | 4.40 | 10.43 |
| CV (%) | 11.12 | 8.90 | 10.97 | 10.99 | 8.25 | 6.53 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
