| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This REG3G Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
REG3G |
| Gene ID: |
130120 |
| Uniprot ID: |
REG3G_HUMAN |
| Specificity: |
This kit recognizes Human REG3 Gamma in samples. No significant cross-reactivity or interference between Human REG3 Gamma and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity. |
| Function | Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. Acts as a hormone in response to different stimuli like anti-inflammatory signals, such as IL17A, or gut microbiome. Is secreted by different cell types to activate its receptor EXTL3 and induce cell specific signaling pathways. Induced by IL17A in keratinocytes, regulates keratinocyte proliferation and differentiation after skin injury. In parallel, inhibits skin inflammation through the inhibition of inflammatory cytokines such as IL6 and TNF. Induced by IL22 in lung epithelial cells, inhibits cytokine production and regulates allergic airway inflammation. Induced in small intestine by inulin-enriched diet and Lactobacillus gasseri enriched microbiome, plays a role in the improvement of gut barrier function, the regulation of energy balance and glucose levels. Modulates microbiota composition in duodenal contents. Produced by nociceptor in response to endotoxins, prevents endotoxic death by targeting kynurenine pathway in microglia. Regenerating islet-derived protein 3-gamma 16.5 kDa form: Has bacteriostatic activity. Regenerating islet-derived protein 3-gamma 15 kDa form: Has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. |
| Protein Name | Regenerating Islet-Derived Protein 3-GammaReg-3-GammaPancreatitis-Associated Protein 1bPap-1bPancreatitis-Associated Protein IbPap IbRegenerating Islet-Derived Protein Iii-GammaReg IiiReg Iii-Gamma Cleaved Into - Regenerating Islet-Derived Protein 3-Gamma 16.5 Kda Form - Regenerating Islet-Derived Protein 3-Gamma 15 Kda Form |
| Database Links | Reactome: R-HSA-6803157 |
| Cellular Localisation | SecretedCytoplasm |
| Alternative ELISA Names | Regenerating Islet-Derived Protein 3-Gamma ELISA kitReg-3-Gamma ELISA kitPancreatitis-Associated Protein 1b ELISA kitPap-1b ELISA kitPancreatitis-Associated Protein Ib ELISA kitPap Ib ELISA kitRegenerating Islet-Derived Protein Iii-Gamma ELISA kitReg Iii ELISA kitReg Iii-Gamma Cleaved Into - Regenerating Islet-Derived Protein 3-Gamma 16.5 Kda Form - Regenerating Islet-Derived Protein 3-Gamma 15 Kda Form ELISA kitREG3G ELISA kitPAP1B ELISA kitUNQ429 ELISA kitPRO162 ELISA kit |
| Specificity | This kit recognizes Human REG3γ in samples. No significant cross-reactivity or interference between Human REG3γ and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-104 | 98 |
| EDTA plasma (n=8) | 90-100 | 95 |
| Cell culture media (n=8) | 96-112 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 5.16 | 10.51 | 38.79 | 5.19 | 9.53 | 38.62 |
| Standard deviation | 0.28 | 0.61 | 1.65 | 0.28 | 0.50 | 1.56 |
| CV (%) | 5.43 | 5.80 | 4.25 | 5.39 | 5.25 | 4.04 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
