| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This REG3A Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
REG3A |
| Gene ID: |
5068 |
| Uniprot ID: |
REG3A_HUMAN |
| Specificity: |
This kit recognizes Human REG3 Alpha in samples. No significant cross-reactivity or interference between Human REG3 Alpha and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity. |
| Function | Regenerating islet-derived protein 3-alpha 15 kDa form: Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Binds membrane phospholipids and kills bacteria by forming a hexameric membrane-permeabilizing oligomeric pore. Acts as a hormone in response to different stimuli like anti-inflammatory signals, such as IL17A, or gut microbiome. Secreted by different cell types to activate its receptor EXTL3 and induce cell specific signaling pathways. Induced by IL17A in keratinocytes, regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway. In parallel, inhibits skin inflammation through the inhibition of inflammatory cytokines such as IL6 and TNF. In pancreas, is able to permealize beta-cells membrane and stimulate their proliferation. Regenerating islet-derived protein 3-alpha 16.5 kDa form: Has bacteriostatic activity. |
| Protein Name | Regenerating Islet-Derived Protein 3-AlphaReg-3-AlphaHepatointestinal Pancreatic ProteinHip/PapHuman Proislet PeptideHipPancreatitis-Associated Protein 1Regenerating Islet-Derived Protein Iii-AlphaReg Iii-Alpha Cleaved Into - Regenerating Islet-Derived Protein 3-Alpha 16.5 Kda Form - Regenerating Islet-Derived Protein 3-Alpha 15 Kda Form |
| Database Links | Reactome: R-HSA-6803157 |
| Cellular Localisation | SecretedFound In The Apical Region Of Pancreatic Acinar Cells |
| Alternative ELISA Names | Regenerating Islet-Derived Protein 3-Alpha ELISA kitReg-3-Alpha ELISA kitHepatointestinal Pancreatic Protein ELISA kitHip/Pap ELISA kitHuman Proislet Peptide ELISA kitHip ELISA kitPancreatitis-Associated Protein 1 ELISA kitRegenerating Islet-Derived Protein Iii-Alpha ELISA kitReg Iii-Alpha Cleaved Into - Regenerating Islet-Derived Protein 3-Alpha 16.5 Kda Form - Regenerating Islet-Derived Protein 3-Alpha 15 Kda Form ELISA kitREG3A ELISA kitHIP ELISA kitPAP ELISA kitPAP1 ELISA kit |
| Specificity | This kit recognizes Human REG3α in samples. No significant cross-reactivity or interference between Human REG3α and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-108 | 101 |
| EDTA plasma (n=8) | 86-101 | 92 |
| Cell culture media (n=8) | 86-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 45.66 | 95.20 | 457.22 | 41.71 | 93.94 | 473.81 |
| Standard deviation | 2.34 | 4.10 | 19.16 | 2.17 | 4.46 | 18.53 |
| CV (%) | 5.12 | 4.31 | 4.19 | 5.20 | 4.75 | 3.91 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
