| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human REG1B kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
REG1B |
| Gene ID: |
5968 |
| Uniprot ID: |
REG1B_HUMAN |
| Specificity: |
This kit recognizes Human REG1 Beta in samples. No significant cross-reactivity or interference between Human REG1 Beta and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | All O-linked glycans consist of Gal-GlcNAc-Gal-GalNAc tetrasaccharide core and get elongated (microheterogeneity). |
| Function | Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. |
| Protein Name | Lithostathine-1-BetaPancreatic Stone Protein 2Psp-2Regenerating Islet-Derived Protein 1-BetaReg-1-BetaRegenerating Protein I Beta |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Lithostathine-1-Beta CLIA kitPancreatic Stone Protein 2 CLIA kitPsp-2 CLIA kitRegenerating Islet-Derived Protein 1-Beta CLIA kitReg-1-Beta CLIA kitRegenerating Protein I Beta CLIA kitREG1B CLIA kitPSPS2 CLIA kitREGL CLIA kit |
| Specificity | This kit recognizes Human REG1β in samples. No significant cross-reactivity or interference between Human REG1β and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-101 | 94 |
| EDTA plasma(n=8) | 91-106 | 99 |
| Cell culture media(n=8) | 93-106 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 196.61 | 410.74 | 1494.96 | 186.88 | 385.51 | 1441.87 |
| Standard deviation | 18.03 | 43.29 | 136.94 | 18.87 | 30.76 | 108.14 |
| CV (%) | 9.17 | 10.54 | 9.16 | 10.10 | 7.98 | 7.50 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
