Applications: |
CLIA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
18.75pg/mL |
Detection Limit: |
31.25~2000pg/mL |
Short Description: |
This human RBP1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
RBP1 |
Gene ID: |
5947 |
Uniprot ID: |
RET1_HUMAN |
Specificity: |
This kit recognizes Human RBP1 in samples. No significant cross-reactivity or interference between Human RBP1 and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Tissue Specificity | Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver. |
Function | Cytoplasmic retinol-binding protein. Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis. |
Protein Name | Retinol-Binding Protein 1Cellular Retinol-Binding ProteinCrbpCellular Retinol-Binding Protein ICrbp-I |
Database Links | Reactome: R-HSA-2453864Reactome: R-HSA-2453902Reactome: R-HSA-975634 |
Cellular Localisation | CytoplasmLipid Droplet |
Alternative CLIA Names | Retinol-Binding Protein 1 CLIA kitCellular Retinol-Binding Protein CLIA kitCrbp CLIA kitCellular Retinol-Binding Protein I CLIA kitCrbp-I CLIA kitRBP1 CLIA kitCRBP1 CLIA kit |
Specificity | This kit recognizes Human RBP1 in samples. No significant cross-reactivity or interference between Human RBP1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 100-117 | 107 |
EDTA plasma(n=8) | 96-110 | 101 |
Cell culture media(n=8) | 93-107 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 97.84 | 201.37 | 899.82 | 90.49 | 195.37 | 893.92 |
Standard deviation | 9.64 | 22.11 | 91.33 | 9.29 | 22.70 | 79.74 |
CV (%) | 9.85 | 10.98 | 10.15 | 10.27 | 11.62 | 8.92 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance