• Human RAD23B (RAD23 Homolog B) Sandwich ELISA Kit (STJE0011810)

Human RAD23B (RAD23 Homolog B) Sandwich ELISA Kit (STJE0011810)

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STJE0011810

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.052ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This RAD23B Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: RAD23B
Gene ID: 5887
Uniprot ID: RD23B_HUMAN
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity
Post Translational Modifications
Function Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with CETN2 appears to stabilize XPC. May protect XPC from proteasomal degradation. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER.it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.
Protein Name Uv Excision Repair Protein Rad23 Homolog B
Hr23b
Hhr23b
Xp-C Repair-Complementing Complex 58 Kda Protein
P58
Database Links Reactome: R-HSA-532668
Reactome: R-HSA-5689877
Reactome: R-HSA-5696394
Reactome: R-HSA-5696395
Cellular Localisation Nucleus
Cytoplasm
The Intracellular Distribution Is Cell Cycle Dependent
Localized To The Nucleus And The Cytoplasm During G1 Phase
Nuclear Levels Decrease During S-Phase
Upon Entering Mitosis
Relocalizes In The Cytoplasm Without Association With Chromatin
Alternative ELISA Names Uv Excision Repair Protein Rad23 Homolog B ELISA kit
Hr23b ELISA kit
Hhr23b ELISA kit
Xp-C Repair-Complementing Complex 58 Kda Protein ELISA kit
P58 ELISA kit
RAD23B ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance