• Human PTX3 (Pentraxin-related protein PTX3) Sandwich ELISA (STJE0004093)

Human PTX3 (Pentraxin-related protein PTX3) Sandwich ELISA (STJE0004093)

SKU:
STJE0004093

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.12pg/mL
Detection Limit: 6.86~5000pg/mL
Short Description: This PTX3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: PTX3
Gene ID: 5806
Uniprot ID: PTX3_HUMAN
Specificity: This kit recognizes Human PTX 3/TSG-14 in samples. No significant cross-reactivity or interference between Human PTX 3/TSG-14 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.
Protein Name Pentraxin-Related Protein Ptx3
Pentaxin-Related Protein Ptx3
Tumor Necrosis Factor Alpha-Induced Protein 5
Tnf Alpha-Induced Protein 5
Tumor Necrosis Factor-Inducible Gene 14 Protein
Tsg-14
Database Links Reactome: R-HSA-6798695
Cellular Localisation Secreted
Alternative ELISA Names Pentraxin-Related Protein Ptx3 ELISA kit
Pentaxin-Related Protein Ptx3 ELISA kit
Tumor Necrosis Factor Alpha-Induced Protein 5 ELISA kit
Tnf Alpha-Induced Protein 5 ELISA kit
Tumor Necrosis Factor-Inducible Gene 14 Protein ELISA kit
Tsg-14 ELISA kit
PTX3 ELISA kit
TNFAIP5 ELISA kit
TSG14 ELISA kit
Specificity This kit recognizes Human PTX3/TSG-14 in samples. No significant cross-reactivity or interference between Human PTX3/TSG-14 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-102 93
EDTA plasma (n=8) 90-104 96
Cell culture media (n=8) 86-102 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 230.55 565.35 2010.46 209.00 617.46 1987.07
Standard deviation 13.63 24.99 96.70 13.63 29.64 104.52
CV (%) 5.91 4.42 4.81 6.52 4.80 5.26
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance