| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.25ng/mL |
| Detection Limit: |
0.781-50ng/mL |
| Short Description: |
This PTPLA Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
HACD1 |
| Gene ID: |
9200 |
| Uniprot ID: |
HACD1_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Isoform 1 is highly expressed in the myocardium, and to a lesser extent in skeletal and smooth muscular tissues including those from stomach, jejunum, and bladder. Also detected in gingival fibroblasts, periodontal ligament cells, osteoblasts and cementoblasts. Isoform 2 is specifically expressed by cementoblasts but also detected in periodontal ligament cells, heart, liver and kidney (at protein level). |
| Post Translational Modifications | N-glycosylated. |
| Function | Isoform 1: Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Isoform 2: In tooth development, may play a role in the recruitment and the differentiation of cells that contribute to cementum formation. May also bind hydroxyapatite and regulate its crystal nucleation to form cementum. |
| Protein Name | Very-Long-Chain3r-3-Hydroxyacyl-Coa Dehydratase 13-Hydroxyacyl-Coa Dehydratase 1Hacd1Cementum-Attachment ProteinCapProtein-Tyrosine Phosphatase-Like Member A |
| Database Links | Reactome: R-HSA-75876 |
| Cellular Localisation | Isoform 1: Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative ELISA Names | Very-Long-Chain ELISA kit3r-3-Hydroxyacyl-Coa Dehydratase 1 ELISA kit3-Hydroxyacyl-Coa Dehydratase 1 ELISA kitHacd1 ELISA kitCementum-Attachment Protein ELISA kitCap ELISA kitProtein-Tyrosine Phosphatase-Like Member A ELISA kitHACD1 ELISA kitPTPLA ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
