| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human PTF1A kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PTF1A |
| Gene ID: |
256297 |
| Uniprot ID: |
PTF1A_HUMAN |
| Specificity: |
This kit recognizes Human PTF1 Alpha in samples. No significant cross-reactivity or interference between Human PTF1 Alpha and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers. |
| Function | Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates. |
| Protein Name | Pancreas Transcription Factor 1 Subunit AlphaClass A Basic Helix-Loop-Helix Protein 29Bhlha29Pancreas-Specific Transcription Factor 1aBhlh Transcription Factor P48P48 Dna-Binding Subunit Of Transcription Factor Ptf1Ptf1-P48 |
| Database Links | Reactome: R-HSA-210747 |
| Cellular Localisation | NucleusCytoplasmIn Chronic Pancreatitis Associated With Pancreas Cancer Preferentially Accumulates In The Cytoplasm Of Acinar/Ductular ComplexesIn The Cytoplasm Loses Its Ability To Form The Ptf1 Complex |
| Alternative CLIA Names | Pancreas Transcription Factor 1 Subunit Alpha CLIA kitClass A Basic Helix-Loop-Helix Protein 29 CLIA kitBhlha29 CLIA kitPancreas-Specific Transcription Factor 1a CLIA kitBhlh Transcription Factor P48 CLIA kitP48 Dna-Binding Subunit Of Transcription Factor Ptf1 CLIA kitPtf1-P48 CLIA kitPTF1A CLIA kitBHLHA29 CLIA kitPTF1P48 CLIA kit |
| Specificity | This kit recognizes Human PTF1α in samples. No significant cross-reactivity or interference between Human PTF1α and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-111 | 101 |
| EDTA plasma(n=8) | 102-115 | 108 |
| Cell culture media(n=8) | 98-110 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 108.88 | 275.43 | 987.00 | 98.76 | 274.35 | 1044.15 |
| Standard deviation | 8.84 | 32.09 | 104.03 | 8.71 | 21.87 | 109.22 |
| CV (%) | 8.12 | 11.65 | 10.54 | 8.82 | 7.97 | 10.46 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
