| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human PRELP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PRELP |
| Gene ID: |
5549 |
| Uniprot ID: |
PRELP_HUMAN |
| Specificity: |
This kit recognizes Human PRELP in samples. No significant cross-reactivity or interference between Human PRELP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Connective tissue. |
| Post Translational Modifications | Glycosylated.contains heparan sulfate. |
| Function | May anchor basement membranes to the underlying connective tissue. |
| Protein Name | ProlarginProline-Arginine-Rich End Leucine-Rich Repeat Protein |
| Database Links | Reactome: R-HSA-2022854Reactome: R-HSA-2022857Reactome: R-HSA-3656225Reactome: R-HSA-3656243Reactome: R-HSA-3656244 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Prolargin CLIA kitProline-Arginine-Rich End Leucine-Rich Repeat Protein CLIA kitPRELP CLIA kitSLRR2A CLIA kit |
| Specificity | This kit recognizes Human PRELP in samples. No significant cross-reactivity or interference between Human PRELP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-106 | 100 |
| EDTA plasma(n=8) | 87-102 | 93 |
| Cell culture media(n=8) | 99-112 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 210.96 | 492.61 | 1838.33 | 204.50 | 491.90 | 1990.48 |
| Standard deviation | 22.26 | 53.94 | 181.08 | 16.48 | 56.17 | 222.14 |
| CV (%) | 10.55 | 10.95 | 9.85 | 8.06 | 11.42 | 11.16 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
