Human PPP1R1A (Protein Phosphatase 1 Regulatory Subunit A) Sandwich ELISA (STJE0019836)

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STJE0019836

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This PPP1R1A Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: PPP1R1A
Gene ID: 5502
Uniprot ID: PPR1A_HUMAN
Sample Type: serum, plasma and other biological fluids
Function Inhibitor of protein-phosphatase 1. This protein may be important in hormonal control of glycogen metabolism. Hormones that elevate intracellular cAMP increase I-1 activity in many tissues. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases.
Protein Name Protein Phosphatase 1 Regulatory Subunit 1a
Protein Phosphatase Inhibitor 1
I-1
Ipp-1
Alternative ELISA Names Protein Phosphatase 1 Regulatory Subunit 1a ELISA kit
Protein Phosphatase Inhibitor 1 ELISA kit
I-1 ELISA kit
Ipp-1 ELISA kit
PPP1R1A ELISA kit
IPP1 ELISA kit
Specificity This kit recognizes Human PPP1R1A in samples. No significant cross-reactivity or interference between Human PPP1R1A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 93
EDTA plasma (n=8) 85-100 91
Cell culture media (n=8) 93-103 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 190.10 558.59 1997.49 186.36 584.37 1844.11
Standard deviation 9.75 27.65 61.92 11.00 27.35 92.94
CV (%) 5.13 4.95 3.10 5.90 4.68 5.04
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance