| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This pNF-H Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
NEFH |
| Gene ID: |
4744 |
| Uniprot ID: |
NFH_HUMAN |
| Specificity: |
This kit recognizes Human pNF-H in samples. No significant cross-reactivity or interference between Human pNF-H and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Protein Name | Neurofilament Heavy PolypeptideNf-H200 Kda Neurofilament ProteinNeurofilament Triplet H Protein |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionAxon |
| Alternative ELISA Names | Neurofilament Heavy Polypeptide ELISA kitNf-H ELISA kit200 Kda Neurofilament Protein ELISA kitNeurofilament Triplet H Protein ELISA kitNEFH ELISA kitKIAA0845 ELISA kitNFH ELISA kit |
| Specificity | This kit recognizes Human pNF-H in samples. No significant cross-reactivity or interference between Human pNF-H and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-99 | 91 |
| EDTA plasma (n=8) | 96-108 | 101 |
| Cell culture media (n=8) | 95-110 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 248.23 | 475.34 | 1765.40 | 238.06 | 485.70 | 1771.88 |
| Standard deviation | 16.46 | 26.71 | 62.50 | 15.28 | 20.35 | 78.32 |
| CV (%) | 6.63 | 5.62 | 3.54 | 6.42 | 4.19 | 4.42 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
