| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human PLVAP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PLVAP |
| Gene ID: |
83483 |
| Uniprot ID: |
PLVAP_HUMAN |
| Specificity: |
This kit recognizes Human PLVAP in samples. No significant cross-reactivity or interference between Human PLVAP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in lung, kidney, heart, aorta, placenta, muscle, pituitary gland, adrenals, mammary gland, bladder, lymph node, bone marrow, trachea, digestive tract, liver and tumor-associated endothelium. |
| Function | Endothelial cell-specific membrane protein involved in the formation of the diaphragms that bridge endothelial fenestrae. It is also required for the formation of stomata of caveolae and transendothelial channels. Functions in microvascular permeability, endothelial fenestrae contributing to the passage of water and solutes and regulating transcellular versus paracellular flow in different organs. Plays a specific role in embryonic development. |
| Protein Name | Plasmalemma Vesicle-Associated ProteinFenestrated Endothelial-Linked Structure ProteinPlasmalemma Vesicle Protein 1Pv-1 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinMembraneCaveolaCytoplasmPerinuclear RegionMembrane-Associated Protein Of CaveolaeFound In Fenestral And Stomatal Diaphragms In Fenestrated Endothelia And Transendothelial ChannelsAlso Colocalized With Cav1 In Perinuclear Region |
| Alternative CLIA Names | Plasmalemma Vesicle-Associated Protein CLIA kitFenestrated Endothelial-Linked Structure Protein CLIA kitPlasmalemma Vesicle Protein 1 CLIA kitPv-1 CLIA kitPLVAP CLIA kitFELS CLIA kitPV1 CLIA kit |
| Specificity | This kit recognizes Human PLVAP in samples. No significant cross-reactivity or interference between Human PLVAP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-101 | 93 |
| EDTA plasma(n=8) | 92-106 | 100 |
| Cell culture media(n=8) | 85-100 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 98.60 | 177.55 | 877.12 | 106.17 | 160.25 | 887.11 |
| Standard deviation | 8.60 | 20.13 | 75.26 | 13.69 | 13.93 | 67.33 |
| CV (%) | 8.72 | 11.34 | 8.58 | 12.89 | 8.69 | 7.59 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
