• Human PLIN2 (Perilipin-2) Sandwich ELISA (STJE0002099)

Human PLIN2 (Perilipin-2) Sandwich ELISA (STJE0002099)

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STJE0002099

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This PLIN2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: PLIN2
Gene ID: 123
Uniprot ID: PLIN2_HUMAN
Specificity: This kit recognizes Human ADRP in samples. No significant cross-reactivity or interference between Human ADRP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Acylated.primarily with C14, C16 and C18 fatty acids. Phosphorylation at Tyr-232 by isoform 1 of CHKA (CHKalpha2) promotes dissociation from lipid droplets: dissociation is followed by recruitment of autophagosome machinery to lipid droplets and subsequent lipid droplet lipolysis.
Function Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.
Protein Name Perilipin-2
Adipophilin
Adipose Differentiation-Related Protein
Adrp
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-9613354
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Cellular Localisation Membrane
Peripheral Membrane Protein
Lipid Droplet
Alternative ELISA Names Perilipin-2 ELISA kit
Adipophilin ELISA kit
Adipose Differentiation-Related Protein ELISA kit
Adrp ELISA kit
PLIN2 ELISA kit
ADFP ELISA kit
Specificity This kit recognizes Human ADRP in samples. No significant cross-reactivity or interference between Human ADRP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 94
EDTA plasma (n=8) 89-105 96
Cell culture media (n=8) 88-103 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.53 1.51 4.21 0.57 1.41 4.21
Standard deviation 0.03 0.07 0.22 0.03 0.08 0.14
CV (%) 5.66 4.64 5.23 5.26 5.67 3.33
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance