• Human PKN1 (Protein Kinase N1) Sandwich ELISA Kit Ready-To-Use (STJE0017897)

Human PKN1 (Protein Kinase N1) Sandwich ELISA Kit Ready-To-Use (STJE0017897)

SKU:
STJE0017897

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.117ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This PKN1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: PKN1
Gene ID: 5585
Uniprot ID: PKN1_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
Post Translational Modifications Autophosphorylated.preferably on serine. Phosphorylated during mitosis. Activated by limited proteolysis with trypsin. (Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation.
Function PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
Protein Name Serine/Threonine-Protein Kinase N1
Protease-Activated Kinase 1
Pak-1
Protein Kinase C-Like 1
Protein Kinase C-Like Pkn
Protein Kinase Pkn-Alpha
Protein-Kinase C-Related Kinase 1
Serine-Threonine Protein Kinase N
Database Links Reactome: R-HSA-5625740
Reactome: R-HSA-5625886
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013149
Cellular Localisation Cytoplasm
Nucleus
Endosome
Cell Membrane
Peripheral Membrane Protein
Cleavage Furrow
Midbody
Associates With Chromatin In A Ligand-Dependent Manner
Localization To Endosomes Is Mediated Via Its Interaction With Rhob
Association To The Cell Membrane Is Dependent On Ser-377 Phosphorylation
Accumulates During Telophase At The Cleavage Furrow And Finally Concentrates Around The Midbody In Cytokinesis
Alternative ELISA Names Serine/Threonine-Protein Kinase N1 ELISA kit
Protease-Activated Kinase 1 ELISA kit
Pak-1 ELISA kit
Protein Kinase C-Like 1 ELISA kit
Protein Kinase C-Like Pkn ELISA kit
Protein Kinase Pkn-Alpha ELISA kit
Protein-Kinase C-Related Kinase 1 ELISA kit
Serine-Threonine Protein Kinase N ELISA kit
PKN1 ELISA kit
PAK1 ELISA kit
PKN ELISA kit
PRK1 ELISA kit
PRKCL1 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance