| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.126ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This PICALM Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
PICALM |
| Gene ID: |
8301 |
| Uniprot ID: |
PICAL_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in all tissues examined. |
| Post Translational Modifications | |
| Function | Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF). |
| Protein Name | Phosphatidylinositol-Binding Clathrin Assembly ProteinClathrin Assembly Lymphoid Myeloid Leukemia Protein |
| Database Links | Reactome: R-HSA-432722Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9696264 |
| Cellular Localisation | Cell MembraneMembraneClathrin-Coated PitGolgi ApparatusCytoplasmic VesicleClathrin-Coated VesicleNucleusColocalized With Clathrin In The Golgi AreaInteraction With Pimreg May Target Picalm To The Nucleus In Some Cells |
| Alternative ELISA Names | Phosphatidylinositol-Binding Clathrin Assembly Protein ELISA kitClathrin Assembly Lymphoid Myeloid Leukemia Protein ELISA kitPICALM ELISA kitCALM ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
