| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This PARC/CCL18 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CCL18 |
| Gene ID: |
6362 |
| Uniprot ID: |
CCL18_HUMAN |
| Specificity: |
This kit recognizes Human PARC/CCL18 in samples. No significant cross-reactivity or interference between Human PARC/CCL18 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The Cys-30/Cys-54 disulfide bond is required for activity. |
| Function | Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. |
| Protein Name | C-C Motif Chemokine 18Alternative Macrophage Activation-Associated Cc Chemokine 1Amac-1Cc Chemokine ParcDendritic Cell Chemokine 1Dc-Ck1Macrophage Inflammatory Protein 4Mip-4Pulmonary And Activation-Regulated ChemokineSmall-Inducible Cytokine A18 Cleaved Into - Ccl18(1-68 - Ccl18(3-69 - Ccl18(4-69 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | C-C Motif Chemokine 18 ELISA kitAlternative Macrophage Activation-Associated Cc Chemokine 1 ELISA kitAmac-1 ELISA kitCc Chemokine Parc ELISA kitDendritic Cell Chemokine 1 ELISA kitDc-Ck1 ELISA kitMacrophage Inflammatory Protein 4 ELISA kitMip-4 ELISA kitPulmonary And Activation-Regulated Chemokine ELISA kitSmall-Inducible Cytokine A18 Cleaved Into - Ccl18(1-68 - Ccl18(3-69 - Ccl18(4-69 ELISA kitCCL18 ELISA kitAMAC1 ELISA kitDCCK1 ELISA kitMIP4 ELISA kitPARC ELISA kitSCYA18 ELISA kit |
| Specificity | This kit recognizes Human PARC/CCL18 in samples. No significant cross-reactivity or interference between Human PARC/CCL18 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-109 | 101 |
| EDTA plasma (n=8) | 86-99 | 91 |
| Cell culture media (n=8) | 88-102 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 250.69 | 450.43 | 2496.99 | 246.20 | 435.82 | 2552.92 |
| Standard deviation | 16.95 | 23.87 | 93.39 | 14.48 | 18.17 | 121.26 |
| CV (%) | 6.76 | 5.30 | 3.74 | 5.88 | 4.17 | 4.75 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
