Human CCL19 (C-C motif chemokine 19) Sandwich ELISA (STJE0004091)

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STJE0004091

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This CCL19 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CCL19
Gene ID: 6363
Uniprot ID: CCL19_HUMAN
Specificity: This kit recognizes Human MIP-3 Beta/ELC/CCL19 in samples. No significant cross-reactivity or interference between Human MIP-3 Beta/ELC/CCL19 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Protein Name C-C Motif Chemokine 19
Beta-Chemokine Exodus-3
Ck Beta-11
Epstein-Barr Virus-Induced Molecule 1 Ligand Chemokine
Ebi1 Ligand Chemokine
Elc
Macrophage Inflammatory Protein 3 Beta
Mip-3-Beta
Small-Inducible Cytokine A19
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-6783783
Cellular Localisation Secreted
Alternative ELISA Names C-C Motif Chemokine 19 ELISA kit
Beta-Chemokine Exodus-3 ELISA kit
Ck Beta-11 ELISA kit
Epstein-Barr Virus-Induced Molecule 1 Ligand Chemokine ELISA kit
Ebi1 Ligand Chemokine ELISA kit
Elc ELISA kit
Macrophage Inflammatory Protein 3 Beta ELISA kit
Mip-3-Beta ELISA kit
Small-Inducible Cytokine A19 ELISA kit
CCL19 ELISA kit
ELC ELISA kit
MIP3B ELISA kit
SCYA19 ELISA kit
Specificity This kit recognizes Human MIP-3β/ELC/CCL19 in samples. No significant cross-reactivity or interference between Human MIP-3β/ELC/CCL19 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-99 95
EDTA plasma (n=8) 94-104 99
Cell culture media (n=8) 94-109 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 22.52 53.02 228.89 22.80 62.03 249.79
Standard deviation 1.21 2.57 9.29 1.22 2.84 10.12
CV (%) 5.37 4.85 4.06 5.35 4.58 4.05
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance